PIERCE，BS3, Bis（Sulfosuccinimidyl） Suberate
- 型號(hào)： 21580
- 更新時(shí)間： 2024-05-31
- 上一個(gè)：21578PIERCE，DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpro
- 下一個(gè)：21585PIERCE，No-Weigh（tm） Format,8 x 2 mg microtubes
訂貨：，，
BS3, Bis（Sulfosuccinimidyl） Suberate介紹
Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
BS3, Bis（Sulfosuccinimidyl） Suberate現(xiàn)貨供應(yīng)
| PIERCE | 21580 | BS3, Bis（Sulfosuccinimidyl） Suberate | 50 MG |
| PIERCE | 21585 | BS3, No-Weigh（tm） Format, 8 x 2 mg microtubes | 8 X 2 MG |
| PIERCE | 21627 | Streptavidin, R-Phycoerythrin Conjugated, 1 mL. | 1 ML |
BS3, Bis（Sulfosuccinimidyl） Suberate品牌介紹
美國(guó)Pierce公司（www.pierce.com）是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html
主要產(chǎn)品包括：
1）化學(xué)發(fā)光體和比色底物
2）分析試劑
3）熒光標(biāo)記探針
4）酶聯(lián)免疫分析試劑盒等
BS3, Bis（Sulfosuccinimidyl） Suberate僅用于科研
Thermo Scientific Pierce BS3 （Sulfo-DSS） is bis（sulfosuccinimidyl）suberate, an amine-to-amine crosslinker that is homobifunctional, water-soluble, non-cleavable and membrane impermeable.
BS3 contains an amine-reactive N-hydroxysulfosuccinimide （NHS） ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.
Because it contains the hydrophilic sulfonyl moiety, BS3 crosslinker is soluble up to ~10mM in water and many commonly used buffers, thus avoiding the use of organic solvents which may perturb protein structure. DSS, the non-water soluble analog of BS3 is also available for applications that require a less hydrophilic crosslinker （e.g., to effect intracellular crosslinking）. DSS and BS3 have essentially identical crosslinking activity toward primary amines.
BS3 Highlights
- Amine-reactive Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- BS3 is water-soluble, so crosslinking can be done in physiologic solutions
- Water-soluble crosslinking reagent is ideal for cell-surface crosslinking
- High-purity, crystalline BS3 can be used to create high-purity crosslinked conjugates