PIERCE，DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpro
- 型號(hào)： 21578
- 更新時(shí)間： 2024-05-31
- 上一個(gè)：21555PIERCE，DISUCCINIMIDYL SUBERATE （DSS）
- 下一個(gè)：21580PIERCE，BS3, Bis（Sulfosuccinimidyl） Suberate
訂貨：，，
DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpropionate）介紹
Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白：蛋白間相互作用，衡量親和層析后的組分含量，估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。
DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpropionate）現(xiàn)貨供應(yīng)
| PIERCE | 21578 | DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpropionate） | 50 MG |
| PIERCE | 21580 | BS3, Bis（Sulfosuccinimidyl） Suberate | 50 MG |
| PIERCE | 21585 | BS3, No-Weigh（tm） Format, 8 x 2 mg microtubes | 8 X 2 MG |
DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpropionate）品牌介紹
美國(guó)Pierce公司（www.pierce.com）是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html
主要產(chǎn)品包括：
1）化學(xué)發(fā)光體和比色底物
2）分析試劑
3）熒光標(biāo)記探針
4）酶聯(lián)免疫分析試劑盒等
DTSSP, 3,3'-Dithiobis （Sulfosuccinimidylpropionate）僅用于科研
Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.
3,3´-Dithiobis（sulfosuccinimidylpropionate） （DTSSP） contains an amine-reactive N-hydroxysulfosuccinimide （sulfo-NHS） ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine （K） residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker. BS3, the non-cleavable analog of DTSSP is also available for applications that require a stable spacer arm that cannot be cleaved in the presence of reducing agents.
DTSSP Highlights
- Amine reactive sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
- Disulfide bond in the spacer arm is readily cleaved by 10-50 mM DTT or TCEP at pH 8.5
- Spacer arm is also cleaved with 5% β-mercaptoethanol in SDS-PAGE sample loading buffer at 100°C for 5 minutes
- Cleavable crosslinker allows separation of crosslinked products
- DTSSP crosslinker is not membrane permeable, so cell-surface crosslinking can be done