重組人干擾素- alpha 2a（rHuIFN-a 2a）
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- 更新時(shí)間： 2024-05-23
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產(chǎn)品介紹
| CYT-204 | 重組人干擾素- alpha 2a（rHuIFN-a 2a） | 20μg/100μg/1mg | Prospec |
| Background: | |
| At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. | |
 | Description : |
| Recombinant Human IFN-alpha 2a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. Interferon-alpha 2a gene was obtained from human leukocytes. The IFN-a is purified by proprietary chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| Recombinant IFN-a is lyophilized from （1mg/ml） solution containing 7.21 sodium chloride and 0.77mg ammonium acetate. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized IFN-alpha-2a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized IFN-alpha-2a although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution IFN-alpha-2a should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please avoid freeze-thaw cycles. | |
| Purity: |
| Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln, conforming to the sequence of native human IFN-alpha . N-terminal methionine has been compley removed enzymatically. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| ProSpec's IFN-alpha 2a is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 2.7 x 108. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of rHuIFN-α 2a. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.924 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of IFN-alpha（2a） as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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