邢台夜先影视文化发展有限公司

你的位置:首頁(yè) >產(chǎn)品展示 > > 以色列Prospec細(xì)胞因子 > 重組人表皮生長(zhǎng)因子(rHuEGF)

重組人表皮生長(zhǎng)因子(rHuEGF)

佰易聚生物商城提供美國(guó)光譜醫(yī)學(xué)透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優(yōu)質(zhì)的分子生物學(xué)試劑和試劑盒、培養(yǎng)基本和培養(yǎng)耗材、細(xì)胞因子和細(xì)胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購(gòu)!
  • 在線留言
產(chǎn)品介紹

 

CYT-217重組人表皮生長(zhǎng)因子(rHuEGF) 100μg/500μg Prospec

 

 

Background:
 A neurotrophic factor that promotes the survival of various neuronal cell types and may play an important role in the injury response in the nervous system.
 
Description :
 Recombinant Human Epidermal Growth Factor produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton.
The EGF is purified by proprietary chromatographic techniques.
 
Physical Appearance:
 Sterile Filtered White lyophilized (freeze-dried) powder.
 
Formulation:
 Recombinant EGF was lyophilized from a concentrated (1mg/ml) solution with no additives.
 
Solubility:
 It is recommended to reconstitute the lyophilized EGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
 
Stability:
 Lyophilized EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution EGF should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
 
Purity:
 Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
 
Amino acid sequence:
 The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native human EGF. N-terminal methionine has been compley removed enzymatically.
 
Dimers and aggregates:
 Less than 1% as determined by silver-stained SDS-PAGE gel analysis.
 
Biological Activity:
 ProSpec's recombinant Human Epidermal Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is less then 0.1 ng/ml corresponding to a specific activity of 1 x 107 Units/mg.
 
Endotoxin:
 Less than 0.1 ng/µg (IEU/µg) of recombinant EGF .
 
Protein content:
 Protein quantitation was carried out by two independent methods:

1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (InliGenetics).
2. Analysis by RP-HPLC, using a standard solution of human EGF as a Reference Standard.
 
Usage:
 Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.


佰易聚生物商城提供美國(guó)光譜醫(yī)學(xué)透析袋,wako生化試劑,amresco生化試劑,sigma生化試劑,優(yōu)質(zhì)的分子生物學(xué)試劑和試劑盒、培養(yǎng)基本和培養(yǎng)耗材、細(xì)胞因子和細(xì)胞分離液、抗體和elisa試劑盒及常用生物試劑等,大量現(xiàn)貨火爆*,歡迎登錄 www.ebioeasy.com或者,,洽談選購(gòu)!


 



更多產(chǎn)品
在線留言

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
禹城市| 抚松县| 耒阳市| 龙泉市| 清水县| 泸水县| 会同县| 克山县| 永和县| 定兴县| 志丹县| 泊头市| 获嘉县| 拜城县| 伽师县| 新沂市| 常熟市| 凌源市| 潜山县| 民丰县| 虞城县| 东光县| 青河县| 乐安县| 吉林省| 洪江市| 兰西县| 石阡县| 沙洋县| 禹城市| 涪陵区| 平潭县| 丘北县| 东丽区| 依安县| 中方县| 承德县| 金沙县| 大姚县| 宁远县| 溧阳市|