重組人可溶性CD40配體/TRAP （rHusCD40L/TRAP）
- 型號：
- 更新時間： 2024-05-23
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產品介紹
| CYT-245 | 重組人可溶性CD40配體/TRAP （rHusCD40L/TRAP） | 10μg/50μg/1mg | Prospec |
| Background: | |
| CD40L or CD154 is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines . CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also costimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma , TNF-alpha , and IL2 . | |
 | Description : |
| Recombinant Human CD40L produced in E.Coli is a non-glycosylated, Polypeptide chain containing 149 amino acids and having a molecular mass of 16308 Dalton. Recombinant CD40L is purified by proprietary chromatographic techniques. | |
| Physical Appearance: |
| Sterile Filtered White lyophilized （freeze-dried） powder. | |
| Formulation: |
| CD40L is lyophilized from a sterile concentrated solution （1mg/ml） with 10mM Sodium Phosphate pH=7.5. | |
| Solubility: |
| It is recommended to reconstitute the lyophilized CD40L in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. | |
| Stability: |
| Lyophilized CD40L although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution CD40L should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles. | |
| Purity: |
| Greater than 98.0% as determined by: （a） Analysis by RP-HPLC. （b） Anion-exchange FPLC. （c） Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. | |
| Amino acid sequence: |
| The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Lys-Gly-Asp. | |
| Dimers and aggregates: |
| Less than 1% as determined by silver-stained SDS-PAGE gel analysis. | |
| Biological Activity: |
| ProSpec's CD40L is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB （Peripheral Mononuclear） cells was found to be 5-10 ng/ml. | |
| Endotoxin: |
| Less than 0.1 ng/µg （IEU/µg） of soluble CD40L. | |
| Protein content: |
| Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.1 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）. 2. Analysis by RP-HPLC, using a standard solution of soluble CD40L as a Reference Standard. | |
| Usage: |
| Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
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