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PIERCE,Ellman's Reagent, 5,5'-Dithio-bis-(2-nitrob

貨號:22582,名稱: Ellman's Reagent, 5,5'-Dithio-bis-(2-nitrobenzoic Acid) ,規(guī)格:5 GM
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Ellman's Reagent, 5,5'-Dithio-bis-(2-nitrobenzoic Acid)介紹


Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。


Ellman's Reagent, 5,5'-Dithio-bis-(2-nitrobenzoic Acid)現(xiàn)貨供應(yīng)



PIERCE

22582

Ellman's Reagent, 5,5'-Dithio-bis-(2-nitrobenzoic Acid)

5 GM

PIERCE

22585

DITHIOBIS (SUCCINIMIDYL PROPIONATE)   (DSP)         1 GM

1 GM

PIERCE

22586

DSP (Lomant's Reagent), 50 mg

50 MG


Ellman's Reagent, 5,5'-Dithio-bis-(2-nitrobenzoic Acid)品牌介紹


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Thermo Scientific Pierce DTNB (Ellman's Reagent) reacts with sulfhydryl groups to yield a colored product, providing a reliable method to measure reduced cysteines and other free sulfhydryls in solution.

Ellman's Reagent (5,5'-dithio-bis-[2-nitrobenzoic acid]) is used to estimate sulfhydryl groups in a sample by comparing to a standard curve of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups can be assayed using the extinction coefficient of TNB (14,150M-1cm-1 at 412nm). Ellman's Reagent has been used for applications other than sulfhydryl estimation, such as for determining alkylthiols by HPLC using a pre-column derivatization procedure and to study thiols in the active site of several enzymes.

Highlights:

  • Determine availability of sulfhydryls for labeling or crosslinking applications
  • Assay by measuring the absorbance at 412nm or using cysteine as a standard
  • Temporarily block sulfhydryls to perform chemistries on other protein functionalities


 Ellman's Reagent, 5,5'-Dithio-bis-(2-nitrobenzoic Acid)僅用于科研


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